Clonexpress one step cloning kit c115
WebJan 15, 2024 · Phanta super-Fidelity DNA Polymerase, Rapid Taq Master Mix and ClonExpress Ultra One Step Cloning Kit C115 from Vazyme Biotech Co., ltd (Nanjing, China). The restriction enzyme Dpn I from ThermoFisher Scientific Co., ltd (Beijing, China). The plasmid miniprep kit, gel extraction kit and PCR cleanup kit were provided by … WebJun 17, 2024 · Application Note: ClonExpress Ultra One Step Cloning Kit17 June 2024. Application Note: ClonExpress Ultra One Step Cloning Kit. In this application note, …
Clonexpress one step cloning kit c115
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WebClonExpress UItra One Step Cloning kit (Vazyme, #C115) is a novel cloning kit, applicable for homologous recombination with 1 - 5 fragments. The Kit is independent of DNA ligase, significantly reducing the self … WebProduct Description. ClonExpress II One Step Cloning (Vazyme, #C112) is a simple, fast, and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of …
WebMar 14, 2024 · Download PDF Copy. ClonExpress ® technology is a fast, simple and efficient DNA seamless cloning technology, enabling the insert of any position of any … WebClonExpress Ultra One Step Cloning Kit: C115-01/02: ClonExpress MultiS One Step Cloning Kit: C113-01/02: ClonExpress II One Step Cloning Kit: C112-01/02: ... ClonExpress II One-Step Cloning Kit uses a highly efficient homologous recombination technology to clone DNA products to very high efficiency in a simple rapid one-step …
WebDec 31, 2024 · ClonExpress II One Step Cloning Kit C112. Cat. No : C112-01: 25 rxn C112-02: 50 rxn. ClonExpress II One Step Cloning is a simple, fast, and high efficient cloning technology and enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. WebFeb 9, 2024 · following the instruction of the ClonExpress II One Step Cloning Kit (Vazyme, China), generating mini-CTX-Ptat-lacZ. The I-F cas operon including its native promoter (8.693-kb) was obtained by PCR employing PA154197 genomic DNA as the template. The DNA fragment was inserted into the KpnI site in the mini-CTX-Ptat-lacZ …
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WebWe offer a complete line of best-in-class reagents to fit every cloning project. With streamlined methods and high-performance reagents, you can easily move forward in your research. Utilize the power of In-Fusion Cloning kits to perform ligation-independent cloning of PCR products into any vector, at any site of linearization. flying upload kdp pricingWebProduct Introduction. ClonExpress Ultra One Step Cloning Kit is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. The unique ligase-independent system of … flying upload loginWebJul 27, 2024 · The gRNA expression box containing the MS2 stem-loop was subcloned from LentiSAM V2 (Puro) by ClonExpress Ultra One Step Cloning Kit (C115, Vazyme). Thus, the CRISPR-Cas9 complex could recruit the MS2-HNRNPK, MS2-DNMT1, or MS2-SETDB1 by the MS2 stem-loop to the promoter of TRAF3IP2. GeneChem Technology constructed … green mountain french toastWebClonExpress Ultra One Step Cloning Kit是新一代的重组克隆试剂盒，可快速高效的将1-5个片段定向克隆至任意载体的任意位点，高度优化的2 × ClonExpress Mix，50℃反应5-15 min完成重组，操作简单快捷。. 组分. C115-01 (25 rxns) C115-02 (50 rxns) 2 × ClonExpress Mix. 125 μl. 2 × 125 μl. pCE ... flying united with kidsWebIt is suitable for targeted cloning to almost any vector at any site. It can efficiently clone 50 bp - 10 kb fragments and carry out one step multi-fragment seamless cloning. Enzyme … flying united with a catWebIi One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... Clonexpress Ii One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 86/100, based on 1 ... green mountain french roast ground coffeeWebClonExpress® Ultra One Step Cloning Kit is a new generation of recombinant cloning kit, which can quickly and efficiently clone 1-5 fragments to any position of any vector. The … flying upload price