WebNov 22, 2024 · Reactive Oxygen Species (ROS) Assay Intracellular hydroperoxide and oxidative stress were determined by flow cytometry using H2-DCF-DA. B16F10 cells (1 × 10 6 /mL) were incubated at 37 °C in a 5% CO 2 incubator. WebThe OxiSelect™ ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, …
Intracellular ROS Assay - Cell Biolabs
WebApr 3, 2024 · The ROS generation was monitored through DCF assay to indicate the action mechanisms of Se@AM. As shown in Figure 11A, the ROS generation by H1N1 influenza virus was increased to 420%. AM and SeNPs slightly inhibited the ROS generation to 280% and 230%. However, Se@AM remarkably decreased the rate of ROS generation (130%). WebMar 8, 2024 · Reactive oxygen species (ROS) in the whole body homogenates were measured using fluorescent dye 2′,7′-dichlorofluorescein diacetate (H 2 DCF-DA, ≥97%, Sigma Aldrich, Gewerbegebiet, Germany) . The reaction mixture contained 1 × PBS (pH 7.4), 50 μM H 2 DCF-DA and 10 μL of body homogenate with a final volume of 200 µL. … ge to pdf
Detection of Total Reactive Oxygen Species in Adherent …
WebDCF Staining Assay for Measurement of ROS. Intracellular ROS levels were measured by ROS assay according to the manufacturers’ standard protocols. Briefly, 1–2 × 10 6 cells were cultured in FCS-free medium with 10 μM dichlorofluorescein diacetate (DCFH-DA) at 37°C for 30 min and were then washed three times with FCS-free medium prior to ... WebSep 22, 2024 · 1. Incubate the drug-treated cells with 0.5 mM of 2,7–dichlorofluorescin diacetate (DCF-DA, Sigma) in a serum-free medium for 15~20 min depending on the cell lines. NB: This should be performed ... WebMay 13, 2010 · After passing through the plasma membrane, this lipophilic and non-fluorescent compound is de-esterified to a hydrophilic alcohol [H (2)DCF … christmas toys for dogs uk